Lab occupant using a minION

MinION Analysis and Reference Consortium: Phase 1 data release and analysis

The advent of a miniaturized DNA sequencing device with a high-throughput contextual sequencing capability embodies the next generation of large scale sequencing tools. The MinION™ Access Programme (MAP) was initiated by Oxford Nanopore Technologies™ in April 2014, giving public access to their USB-attached miniature sequencing …

Two horizontally stacked fuzzy double helixs

Sequence progressive alignment, a framework for practical large-scale probabilistic consistency alignment

Motivation: Multiple sequence alignment is a cornerstone of comparative genomics. Much work has been done to improve methods for this task, particularly for the alignment of small sequences, and especially for amino acid sequences. However, less work has been done in making promising methods that …

Backlit dna exhibit

Trawler: de novo regulatory motif discovery pipeline for chromatin immunoprecipitation.

We developed Trawler, the fastest computational pipeline to date, to efficiently discover over-represented motifs in chromatin immunoprecipitation (ChIP) experiments and to predict their functional instances. When we applied Trawler to data from yeast and mammals, 83% of the known binding sites were accurately called, often …

Medaka fish

The discovery, positioning and verification of a set of transcription-associated motifs in vertebrates

We have developed several new methods to investigate transcriptional motifs in vertebrates. We developed a specific alignment tool appropriate for regions involved in transcription control, and exhaustively enumerated all possible 12-mers for involvement in transcription by virtue of their mammalian conservation. We then used deeper …